LIME JUICE-ANTI PANCREATIC CANCER PER RESEARCH IN USA.

Lime Juice Inhibits 89% of Pancreatic Cancer Cells In Vitro: In this lab study, four different extracts of Mexican lime juice (citrus aurantifolia) were all found to potently inhibit the growth of human ‪cells (Panc-28) in vitro, up to 89% after 72 hours of treatment. This was due in part to direct killing of the cancer cells via programmed cell death (apoptosis). The lime juice also showed powerful free-radical scavenging activity. Several key compounds were identified in the ‬ juice, such as rutin, neohesperidin, hesperidin and hesperitin as well as several limonoids. In real studies on humans, citrus fruits have often been shown to impart significant risk reductions for pancreatic cancer but limes are not consumed enough to be categorized alone. One recent study showed that those consuming 2-3 servings daily of citrus / melon / berries had a 30% reduced risk for pancreatic cancer, while those drinking 200-300 grams of orange or grapefruit juice had an astounding 48% reduced risk of pancreatic cancer (that's just a normal drinking cup worth!). Personally, I consume the freshly squeezed juice of one lemon and one lime every day, and can clearly feel the boost it gives to my energy levels

 

See below-

J Agric Food Chem. 2009 Nov 25;57(22):10933-42. doi: 10.1021/jf901718u.

Bioactive compounds from Mexican lime ( Citrus aurantifolia ) juice induce apoptosis in human pancreatic cells.

Patil JR¹, Chidambara Murthy KN, Jayaprakasha GK, Chetti MB, Patil BS.

Author information

• ¹Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, Texas 77845-2119, USA.

Abstract

Lime (Citrus aurantifolia Swingle) is one of the major citrus fruits and widely consumed, but there is limited evidence about its health-promoting properties. Hence, an investigation was conducted to understand the chemopreventive effects of lime juice on pancreatic cancer cells and the possible mechanism for induction of apoptosis using Panc-28 cells. Freeze-dried lime juice was extracted with different solvents, such as chloroform, acetone, MeOH, and MeOH/water (8:2). The chloroform extract showed the highest (85.4 and 90%) radical-scavenging activity by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) methods at 624 microg/mL, whereas the MeOH/water extract showed the lowest (<20%) activity. The active components were identified by high-performance liquid chromatography (HPLC) using a C-18 column as rutin, neohesperidin, hesperidin, and hesperitin. Furthermore, the limonoids identified are limonexic acid, isolimonexic acid, and limonin. All of the extracts of lime juice inhibited Panc-28 cancer cell growth. The MeOH extract exhibited the maximum activity, with an IC50 value of 81.20 microg/mL after 72 h. The inhibition of Panc-28 cells was in the range of 73-89%, at 100 microg/mL at 96 h. The involvement of apoptosis in induction of cytotoxicity was confirmed by expression of Bax, Bcl-2, casapase-3, and p53. The results of the present study clearly indicate that antioxidant activity is proportionate to the content of flavonoids and proliferation inhibition ability is proportionate to the content of both flavonoids and limonoids.

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