Abstract Title:
Apoptosis induction of Persicae Semen extract in human promyelocytic leukemia
(HL-60) cells.
Abstract Source:
Abstract:
The major ingredient of Persicae Semen is a cynogenic compound, amygdalin
(D-mandelonitrile-beta-gentiobioside). Controversial results on the anticancer
activity of amygdalin were reported due to its conversion to its inactive
isomer, neoamygdalin. In order to inhibit the epimerization of amygdalin, we
used newly developed simple acid boiling method in preparation of Persicae Semen
extract. HPLC analysis revealed most of amygdalin in Persicae Semen extract was
active D-form. Persicae Semen extract was used to analyze its effect on cell
proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60)
cells. Persicae Semen extract was cytotoxic to HL-60 cells with IC50 of 6.4
mg/mL in the presence of 250 nM of beta-glucosidase. The antiproliferative
effects of Persicae Semen extract appear to be attributable to its induction of
apoptotic cell death, as Persicae Semen extract induced nuclear morphology
changes and internucleosomal DNA fragmentation.
Article
Published Date : Feb 01, 2003
Study Type : In Vitro Study
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